ISEV has gone virtual and we've gone with it! Spectradyne is proud to be a Bronze sponsor of the conference, now being held virtually July 20-22. View our poster entitled, "Accurate EV Concentration is Critical for Experimental Rigor and Reproducibility" (Abstract ID: 829670) as well as a variety of presentations from nCS1 users around the world.
Check out how our customers are using Microfluidic Resistive Pulse Sensing (MRPSTM) to make scientific discoveries:
Scientists at John Hopkins Center for Nanomedicine (Baltimore, MD) studying mitochondrial function in retinal pigmented epithelial (RPE) cells used MRPS to quantify differences in EV formation in cells with diminished mitochondrial capacity. The EVs may be a diagnostic biomarker for monitoring the spread of degeneration.
J.Y. Ahn, S. Datta, E. Bandeira, M. Cano, E. Mallick, U. Rai, B. Powell, J. Tian, K.W. Witwer, J.T. Handa, M.E. Paulaitis, "Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria," BBA - General Subjects (in press, 2020). doi: 10.1016/j.bbagen.2020.129598
MRPS was used as a primary tool to investigate and qualify a new method for protein quantification of extracellular vesicles by ATR-FTIR.
V. Szentirmai, A. Wacha, C. Nemeth, D. Kitka, A. Racz, K. Heberger, J. Mihaly, Z. Varga, "Reagent-free total protein quantification of intact extracellular vesicles by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy," Anal. Bioanal. Chem. (2020). doi.org/10.1007/s00216-020-02711-8
In the development of liposomes containing copper for in vitro and in vivo anticancer activity, the same group relied on Spectradyne's MRPS to characterize and quantify their copper complexes for the optimization of the liposomal formulation.
A. Gaal, T. M. Garay, I. Horvath, D. Mathe, D. Szollosi, D. S. Veres, J. Mbuotidem, T. Kovacs, J. Tovari, R. Bergmann, C. Streli, G. Szakacs, J. Mihaly, Z. Varga, N. Szoboszlai, "Development and In Vivo Application of a Water-Soluble Anticancer Copper Ionophore System Using a Temperature-Sensitive Liposome Formulation," Pharmaceutics 12, 466 (2020). doi.org/10.3390/pharmaceutics12050466
MRPS size measurements enable determination of the thickness of the protein corona and hydration layer in extracellular vesicles and synthetic liposomes.
Z. Varga, B. Fehera, D. Kitka, A. Wacha, A. Bota, S. Berenyi, V. Pipich, J.-L. Fraikin, "Size Measurement of Extracellular Vesicles and Synthetic Liposomes: The Impact of the Hydration Shell and the Protein Corona," Colloids and Surfaces B: Biointerfaces (in press, 2020). doi.org/10.1016/j.colsurfb.2020.111053
Tired of waiting days for biological titer to quantify virus? What if you could measure total virus counts in minutes without cell culture? Spectradyne's nCS1 does exactly that-get total virus count in minutes with only 3 microliters of your precious sample. Read our recent blog post for more details here.
Many of you have never seen what it looks like to operate the nCS1, so we put together a short video demonstration. This 13-minute video will walk you through a "virtual demo" of the nCS1: from loading the sample into a microfluidic cartridge to producing a final report. You will see how easy it is to get high quality, high resolution nanoparticle size and concentration data from the nCS1 in just minutes. Once you've seen this video, you will be ready to send us samples to see your own data in high definition!
Spectradyne's nCS1 has been used to quantify a broad range of virus types over the years. From bacteriophage to HIV, here are some examples:
Spectradyne is proud to support its customers who have pivoted in their research focus to address the COVID-19 pandemic. Direct measurements of SARS-CoV-2 virus have been made on the nCS1, and additional studies are planned for the near future — we'll keep you posted. Until then stay safe and let us know how we can help your science!
Spectradyne is a world leader in the microfluidic measurement of nanoparticles.
Nanoparticle analysis using resistive pulse sensing avoids many of the difficulties associated with DLS and optical tracking. Spectradyne is the only company providing microfluidic particle size analysis, now combined with single particle fluorescence.
Check us out today!