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Spectradyne’s ARC™ Particle Analyzer compares membrane dyes for extracellular vesicles: ExoBrite™ True EV Membrane Stain and PKH67

Extracellular vesicles (EVs) derived from HEK-293 cells and expressing human CD81 were fluorescently labeled with membrane dyes, and co-labeled with an antibody marker to evaluate:

  • -The relative staining efficacy between lipophilic membrane dyes ExoBrite™ True EV Membrane Stain and PKH67.
  • -The presence of dye aggregates within the size range of extracellular vesicles.
  • -Compatibility of ExoBrite™ True EV Membrane Stain with antibody staining for co-localization analysis with Spectradyne’s ARC particle analyzer.

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