Virus and Gene Therapy
Rapid viral titer: Absolute virus concentration in minutes, directly in complex media.
Accurate virus concentration and size
Single-particle full vs. empty by fluorescence
Results in minutes, no tissue culture required
See how our F-MRPS technology stacks up against other methods in Virus applications below
Accurate viral vector quantification: Concentration, size & nucleic acid content.
Efficient cell transduction for gene therapy applications depends critically on high quality viral vector preps. But because virus is produced in complex biological systems, direct quantification poses a challenge. Live biological titer can take days to perform, and surface binding methods such as SP-IRIS or ELISA-based protein measurements do not provide accurate concentration. Mass photometry fails for impure samples, which can contain overwhelming concentration of non-viral particles.
What if you could measure the true concentration of intact virus directly from cell culture, in just a few minutes?
Fast & Easy: Virus Size & Concentration with MRPS.
The figure above demonstrates the repeatability of MRPS for viral titer measurements. Human adenovirus 5 was measured in three technical replicates, showing a clear virus population in the sample with mean diameter approximately 85 nm and excellent repeatability. Each measurement required just a few minutes from start to finish, and no need for tissue culture or incubation.
Virus purity and titer in a single measurement.
All biological samples contain heterogeneous populations of many different particle types. Spectradyne’s ARC particle analyzer measures the size and concentration of the entire particle distribution using MRPS, and simultaneously quantifies fluorescent subpopulations of interest using up to 3 different color channels.
In the example above, intact human adenovirus 5 (hAd5) are directly quantified within a complex mixture of off-target biological nanoparticles, in minutes, without tissue culture. hAd5 was mixed with a broad size distribution of extracellular vesicles, and the mixture was stained with a membrane-permeable intercalating dye to illuminate the nucleic acid contained inside the virus. With a single measurement of the stained sample, the concentration of the intact hAd5 is measured to be 5.4 x 109 particles/ml, representing 64% of all particles in the measured size range.
How our MRPS technology stacks up against DLS and NTA
Quantify a broad range of viruses with MRPS.
Is lentivirus more your vector of choice? How about a vector from the Rhabdoviridae family? Here are a few different viruses effectively quantified with Spectradyne’s technology:
- Adeno-associated virus (AAV) aggregates
- Bacteriophage (PR-772, T4, T3, L-VIR)
- Maraba virus
- Herpes Simplex Virus (HSV)
- Human Immunodeficiency Virus (HIV)
- Murine Leukemia Virus (MLV)
- Mouse Hepatitis Virus (MHV)
- Myxoma Virus
Don’t see your favorite viral vector on this list? Reach out to us!