Researchers increasingly turn to Spectradyne's technology to gain a deeper knowledge of their particles, from extracellular vesicles (EVs) to liposomes to viruses. The high-resolution and richness of information delivered by Spectradyne's Microfluidic Pulse Sensing (MRPS) allow scientists to monitor and verify cellular behavior and process development.
Recently, several papers published by scientists at the Research Centre for Natural Sciences (Budapest, Hungary) demonstrate the varied and important roles MRPS plays in many of their studies.
In one study, the size measurements from MRPS enable determination of the thickness of the protein corona and hydration layer in extracellular vesicles and synthetic liposomes.
Z. Varga, B. Fehera, D. Kitka, A. Wacha, A. Bota, S. Berenyi, V. Pipich, J.-L. Fraikin, "Size Measurement of Extracellular Vesicles and Synthetic Liposomes: The Impact of the Hydration Shell and the Protein Corona," Colloids and Surfaces B: Biointerfaces (in press, 2020)
doi.org/10.1016/j.colsurfb.2020.111053
In the development of liposomes containing copper for in vitro and in vivo anticancer activity, the same group relied on Spectradyne's MRPS to characterize and quantify their copper complexes for the optimization of the liposomal formulation.
A. Gaal, T. M. Garay, I. Horvath, D. Mathe, D. Szollosi, D. S. Veres, J. Mbuotidem, T. Kovacs, J. Tovari, R. Bergmann, C. Streli, G. Szakacs, J. Mihaly, Z. Varga, N. Szoboszlai, "Development and In Vivo Application of a Water-Soluble Anticancer Copper Ionophore System Using a Temperature-Sensitive Liposome Formulation," Pharmaceutics 12, 466 (2020)
doi.org/10.3390/pharmaceutics12050466
Most recently, the group employed MRPS as a primary tool to investigate and qualify a new method for protein quantification of extracellular vesicles by ATR-FTIR.
V. Szentirmai, A. Wacha, C. Nemeth, D. Kitka, A. Racz, K. Heberger, J. Mihaly, Z. Varga, "Reagent-free total protein quantification of intact extracellular vesicles by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy," Anal. Bioanal. Chem. (2020)
doi.org/10.1007/s00216-020-02711-8
Scientists at John Hopkins Center for Nanomedicine (Baltimore, MD) studying mitochondrial function in retinal pigmented epithelial (RPE) cells used MRPS to quantify differences in EV formation in cells with diminished mitochondrial capacity. The EVs may be a diagnostic biomarker for monitoring the spread of degeneration.
J.Y. Ahn, S. Datta, E. Bandeira, M. Cano, E. Mallick, U. Rai, B. Powell, J. Tian, K.W. Witwer, J.T. Handa, M.E. Paulaitis, "Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria," BBA - General Subjects (in press, 2020)
doi: 10.1016/j.bbagen.2020.129598
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