Virus Quantification using the nCS1TM


Accurate Viral Titer in Minutes

Conventional methods for quantifying virus such as live biological titer can take days to return results. The modern fast pace of research and current examples such as COVID-19 necessitate faster measurements. Researchers in many areas of virus research including basic virology, gene therapy, and epidemiology are looking for more modern approaches for measuring virus.

Spectradyne's nCS1TM delivers accurate viral particle counts in just a few minutes using Microfluidic Resistive Pulse Sensing (MRPSTM). The technology is fast, easy to adopt and employs disposable microfluidic cartridges for analysis — and it only requires a few microliters of your precious sample for analysis.

nCS1TM key features for virus quantification:

  • Total virus particle count in minutes
  • Disposable analysis cartridges — no laborious cleaning
  • Only uses 3 microliters sample required

instrument

"We eagerly purchased Spectradyne's current MRPS technology for the value it delivers in therapeutic retroviral quantification. We have been impressed with the results it delivers and how practically it fits with our industrial process flow..."

PhD scientist, Gene Therapy Co. Inc.

Better science:

For measuring the infectivity of viruses, testing the immunogenicity of different viral preparations, and almost any down-stream analysis, accurate virus concentration measurements are critical for performing well-controlled experiments.

A retroviral drug product was serially diluted and the concentration measured as a function of size showing the excellent concentration linearity of the nCS1TM:

virus dilution

Serial dilutions of a retroviral drug product accurately quantified by Spectradyne's nCS1TM.

More and more researchers are choosing the nCS1TM for virus quantification because it is fast and easy to use and delivers a more accurate analysis of their virus samples than they can obtain using any other method.


Measure your samples now!

For more information and for how to set up a measurement,
send an email to sales@spectradynellc.com or contact us here.

Higher purity of viral preparations

Accurate and high-resolution particle concentration measurements enable direct quantification of the purity of virus preparations. Because the nCS1TM measures all types of particles in the sample, it can be used to quantify impurities and other non-viral particles in a given preparation.

The figure below shows measurements of a virus preparation at progressive stages of purification. Clearly purification step 2 significantly enriches for the target virus, as evidenced by the peak in the size distribution.

virus enrichment

Quantitative analysis of sample purification steps using the nCS1TM enable better informed decisions and speed up R&D.

Measurement examples

Customers use the nCS1TM to characterize a broad variety of viruses, including:

  • Lentivirus
  • Adenovirus
  • Adeno-associated virus aggregates
  • Bacteriophage (PR-772, T4, T3, L-VIR)
  • Influenza A, B (FluA, FluB)
  • Rabies
  • Herpes Simplex Virus (HSV)
  • Human Immunodeficiency Virus (HIV)
  • Murine Leukemia Virus (MLV)
  • Mouse Hepatitis Virus (MHV)
  • Myxoma Virus

Check out our blog page on interesting virus measurements made with the nCS1TM here

We have detailed measurements on a number of different virus targets. These include measurements of a myxoma virus, a brick-shaped virion of about 200 nm to 300 nm in diameter. In one example these were tested in a cell culture medium with little sample preparation. The nCS1TM was easily able to detect the virus population at 260 nm, even with a high concentration culture media background. Another example is where the nCS1TM was used to measure both a wild-type flu B virus (WT) and a mutant form. A third example is provided by a measurement of maraba virus, which is reported by TEM to have a cylindrical diameter of ~65 nm and a length of ~180 nm. You can read more about these measurements on our blog page here.

Your virus not in this list? Email us to learn more or Send us a sample!


Interested in pricing?

Send an email to sales@spectradynellc.com or
click here to access a quote form.


Spectradyne's nCS1TM delivers the most accurate concentration and size measurements of virus available in a practical bench top solution. The nCS1TM is fast and easy to use, and requires only 3 microliters of your sample to help you do better viral science. Contact us today for a free sample measurement or to request a demonstration!

Read our virus application note,
or send an email to info@spectradynellc.com.

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