At the recent International Society of Extracellular Vesicles (ISEV) 2017 Annual Meeting in Toronto, Jean-Luc Fraikin et al. presented a research paper, entitled Size and concentration determination of extracellular vesicles as small as 50 nm in diameter at a rate beyond 10,000 EV/s. This paper details validation of the nCS1 as a tool for analyzing EVs, as well as comparing it to other available techniques.
We are happy to provide a copy of this presentation on request.
Are you looking for a reliable method to analyze the nanoparticles in your sample? There are two distinct approaches to detecting and measuring the size of nanoparticles in solution, optically-based methods and electrical methods. The only direct way to individually detect and directly measure the size of nanoparticles is using resistive pulse sensing, as developed by Spectradyne LLC. In this method, nanoparticles are made to flow through a microfluidic circuit that includes an engineered nanopore. Each individual particle is detected and its size measured, using a technique that is the gold standard for whole blood cell counting. Spectradyne's team of engineers has brought this clinical standard to the world of nanoparticle analysis, bringing unprecedented precision and accuracy to the world of nanoparticle analysis.
Spectradyne's novel implementation of resistive pulse sensing yields direct diameter measurements of each and every particle, and through its calibrated flow rate measurement, also provides accurate concentration information as a function of particle diameter. This unique combination of high precision individual particle measurements and calibrated concentration makes Spectradyne's nCS1 a unique player in the field of nanoparticle analysis.
Optically-based analysis methods, by contrast, rely on the scattering of light by the nanoparticles of interest. These optically-based techniques do not in fact directly measure the sizes of particles, but instead rely on complex mathematical formulas with many hidden assumptions in order to generate estimates of particle diameter. The amount of light scattered depends strongly on the contrast of the optical index of refraction of the nanoparticles, in comparison to the suspending medium; as a result, particles with different indices will yield different results. Biologically-based nanoparticles often have an index so close to that of water that they are essentially invisible! In addition, the intensity of scattered light varies as the sixth power of the diameter, so that results are often strongly skewed towards these larger particles, making reported distributions inaccurate and unreliable. Furthermore, for the most popular method, known as dynamic light scattering or DLS, the concentration of any particular diameter particle, or of the particle solution as a whole, is not even accessible!
Spectradyne's nCS1 instrument provides a unique platform for the rapid quantitative measurement of nanoparticles in solution. The method used to measure nanoparticles is electrical in nature, so does not rely on an optical index contrast to distinguish particles from their suspending medium. The instrument measures individual nanoparticles and rapidly accumulates statistically-reliable distributions of particle sizes with quantitative concentration information. This unique capability separate the nCS1 from any other instrument on the nanoparticle analysis marketplace.
Watch a video of the nCS1 in action!